RP - HPLC method comparative study of different extraction methods on the content of paeoniflorin from Radix Paeoniae Rubra

Objective To explore an optimal extraction method by comparatively study of five different extraction methods on extraction of paeoniflorin from Radix Paeoniae Rubra.Methods Five extraction processes,soaking extraction,reflux extraction,accelerated solvent extraction method(ASE),ultrasonic extraction and supercritical fluid extraction(SFE),were exploited respectively to extract paeoniflorin from Radix Paeoniae Rubra.To study these extraction methods by HPLC as a reference index of paeoniflorin.Results The content of paeoniflorin of soaking methods was the highest,followed by Reflux extraction,ASE,SFE method,Ultrasonic extraction.Conclusion Paeoniflorin content was quite different from different extraction,the soaking method extracted the highest rate.Results showed that the high temperature and pressure will significantly reduce the extraction effect of paeoniflorin.