Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System

HSV-2 DNA was extracted and used as template in polymerase chain reactions to amplify gD gene.The PCR product was cloned into the donor plasmid pFastBacⅠ.After the transformation and selection,the recombinant plasmid was sequenced.Then,the pFastBacⅠ-gD was introduced into the competent cells E.coli DH10Bac,which containing a shuttel vector,Bacmid.The recombinant bacmid DNA was isolated and transfected into the insect cells Sf9 to produce the first generation recombinant virus.The insect Sf9 cells were infected with the recombinant virus to express the target protein.The expressed protein was detected by SDS-PAGE and Western blot.The gD gene was successfully expressed by Bac-to-Bac Baculovirus Expression System.This work was basic for the future.