Expression and detection of 3D gene of FMDV in recombinant baculovirus

According to the 3D gene of FMDV,the primers of polymerase gene were designed,and the objective fragment was (1 410 bp) which was cloned into pMD18_T vector.Then the 3D gene was cloned into baculovirus expression vectors pFastBacⅠ,and the recombinant plasmid of pFastBac_3D was constructed.The plasmid were transformed to DH10 Bac competent cell,and was screened by three antibiotics and blue_white patch, the white colony was the recombinant Bacmid_3D.And then the bacmid_3D was transfected into the Sf9 insect cells,by three passaging the recombinant baculovirus was obtained.Finally,the expressed productions were detected by SDS_PAGE and Western_blotting,and the results showed that the polymerase(3D protein) was high_efficiently expressed in recombinant baculovirus.