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[Construction and expression of recombinant baculovirus with Schistosoma japonicum SjPP gene].

Li-Xiao Yao, Lihong Tao, Guan-Zhen Yang, Xiang‐Fu Wu, You-Min Cai, Jiaojiao Lin

Abstract

OBJECTIVE: To express the soluble recombinant Schistosoma japonicum SjPP proteins in TN5B1-4 cells. METHODS: The total RNA was extracted from adult worms of Schistosoma japonicum. The whole coding sequence of SjPP gene was synthesized by RT-PCR and cloned into donor plasmid. The recombinant donor pFastBac-SjPP was transformed into E.coli DH10Bac forming Bacmid-SjPP which was transfected into insect cell with cational lipofectin. The fusion protein SjPP was analyzed with SDS-PAGE and Western blotting. RESULTS: The infective recombinant baculovirus Bacmid-SjPP was obtained and SjPP protein was expressed in insect cells. CONCLUSION: The recombinant protein SjPP has been expressed in insect TN5B1-4 cells with proper antigenicity.

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Available abstract

Key concepts: Schistosoma japonicum, Recombinant DNA, Antigenicity, Biology, Molecular biology, Transfection, Plasmid, Gene