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Determination of Avermectin Residues in Eel Tissue by HPLC

Liang Zhen

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Abstract

A rapid HPLC method for determination of avermetion in eel tisue was developed. After extracted by acetonitrile and cleaned up by liquid phase extraction on dichloromethane, concentrated, following derivatization with N-methylimidazole and triflucroacetle, avermection determined by high performance liquid chromatography(HPLC) with fluorescence detection. Recoveries ranged from 78.3%～101.9% for eel tisue samples fortified with avermection ranges from 2～20μg/kg, the limited level of detection is 1μg/kg.

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What this paper is about

A rapid HPLC method for determination of avermetion in eel tisue was developed. After extracted by acetonitrile and cleaned up by liquid phase extraction on dichloromethane, concentrated, following derivatization with N-methylimidazole and triflucroacetle, avermection determined by high performance liquid chromatography(HPLC) with fluorescence detection. Recoveries ranged from 78.3%～101.9% for eel tisue samples fortified with avermection ranges from 2～20μg/kg, the limited level of detection is 1μg/kg.

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Available abstract

A rapid HPLC method for determination of avermetion in eel tisue was developed. After extracted by acetonitrile and cleaned up by liquid phase extraction on dichloromethane, concentrated, following derivatization with N-methylimidazole and triflucroacetle, avermection determined by high performance liquid chromatography(HPLC) with fluorescence detection. Recoveries ranged from 78.3%～101.9% for eel tisue samples fortified with avermection ranges from 2～20μg/kg, the limited level of detection is 1μg/kg.

Key concepts: Chromatography, Avermectin, High-performance liquid chromatography, Chemistry, Derivatization, Dichloromethane, Extraction (chemistry), Acetonitrile

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