Determination of florfenicol residue in eel muscle by HPLC

A method for detecting florfenicol residue in eel muscle was established. Florfenicol residue was extracted from eel muscle with ethyl acetate, followed by sample cleanup with n-hexane and C_(18) solid-phase extraction cartridge, and quantitation by liquid chromatography with DAD detector. The linear range was 1～200 ng and the results showed a good linear relationship (r=0.9999). For spiked samples, the recoveries were above 90%, the relative standard deviations were 4.24%～7.36%,the limit of detection and limit of quantification were 9.1 μg/kg and 22.3 μg/kg, respectively.