Detection of classIintegron in multi-resistant Enterobacter cloacae

Objective To investigate the distribution and effect of classⅠintegron in 40 strains of clinical multi-resistant Enterobacter cloacae.Methods Kirby-Bauer method was used to analyze the drug resistance of 40 strains of multi-resistant Enterobacter cloacae and the drug resistance gene was analyzed by polymerase chain reaction(PCR).Results 28 strains(70.0%)of 40 clinical isolates were observed to contain classⅠintegron.Drug resistance test showed their resistance rate to 10 kind of antibacterials as follow:7.5% to imipenem,5.0% to meropenem,75.0% to piperacillin-tazobactam,55.0% to amikacin,12.5% to cefepime,52.5% to ceftazidime,55.0% to cefotaxime,90.0% to ceftriaxone;81.8% to ticarcillin-clavulanic acid,and 85.0% to ciprofloxacin.Conclusion This study shows that the resistance situation of Enterobacter cloacae isolates is very serious,and classⅠintegron may play an important role in the multidrug resistant mechanism.