Study on the T lymph cell subgroup CD4~+/CD25~+ and CD8~+/CD28~- in the peripheral blood of patients with chronic hepatitis B

Objective To explore the relationship between the CD4~+/CD25~+ and CD8~+/CD28~- regulator lymphocyte in the peripheral blood and the chronic hepatitis B by detecting the level of CD4~+/CD25~+ and CD8~+/CD28~-. Methods CD4~+/CD25~+ and CD8~+/CD28~- regulator lymphocyte were detected by flow cytometry with multicolor fluorescence technology in 280 cases of chronic hepatitis B, 28 cases of HBV asymptomatic carriers (ASC) and 22 healthy volunteers. Results The percent of CD4+/CD25+ lymphocytes of ASC(3.23± 1.37)% was significantly high than that of chronic hepatitis B(1.85± 1.38)% and controls(1.59± 0.54)% . There was no significantly difference between the chronic hepatitis B and that of controls; between the chronic hepatitis B with HBeAg negative( 1.66± 1.09) % and HBeAg positive( 1.96± 1.43) % ; CD8+/ CD28- regulator lymphocyte in patients with chronic hepatitis B(79.53± 16.07)% and ASC(89.30± 5.12)% were significantly high than that of controls(63.93± 7.85)% ; CD8+/ CD28- regulator lymphocyte in patients with HBeAg positive (87.47± 17.82)% was significantly higher than that of HBeAg negative (64.90± 15.26)% . Conclusion The condition was in virus carried of HBV asymptomatic carriers was related to the raising level of CD4+/CD25+ and CD8+/ CD28- regulator lymphocytes.