A substype analyse of peripheral blood CDS lymphocyte in patients with chronic hepatitis virus B

Objective To discuss the relationship between the substype of peripheral blood CD8 lymphocyte in patients with chronic hepatitis virus B and clinic condition and the replication of HBV. Methods CD45RO, CD45RA and CD28 of peripheral blood CD8 lymphocyte were detected by flow cytometry with multicolor fluorescence technology in 107 patients with chronic hepatitis virus B and 20 healthy volunteers. Results The total quantity of CD8 lymphocyte in patientswith chronic hepatitis virus B was not different from that in controls. The percent of CD3+ CD8+/CD45R0+ cells in patients with chronic active hepatitis was significantly higher than that of the control (15.7±3. 35)% vs( 8. 56±1. 12)% , P 0. 05 ] , the percent of CD3+ CD8+ /CD45 RA+ cells was significantly lower than that of the control [ ( 12. 3±4.39)% vs (17. 0±3. 71)% , P 0. 05 ] . The percent of CD3+ CD8+ CD28+ cells in patients with chronic active hepatitis was significantly higher than that of the control [ (23. 7±3. 35)% vs (15. 1±3. 17) % , P 0. 05 ] , The percent of CD3+ CD8+ CD28- cells in patients with chronic taketive hepatitis was significantly higher than that of the control [ ( 16. 3±4. 37) % vs (9. 09±3. 15 ) % P 0. 05 ]. There was no difference of CD3+ CD8+/CD45R0+ cells between the high quantity group and low quantity group, so as the substypes of CD3+ CD8+ /CD45RA+ cells, CD3+ CD8+ CD28+ cells and CD3+ CD8+ CD28- cells. Conclusion The state of chronic hepatitis sufferer was independent of the quantity of CD8/ lymphocyte , it might be dependent on the substypes of CD3+ CD8+/CD45R0+ and CD3+ CD8+ CD28+. But there was no relationship between the substypes of CD8 lymphocyte and the change of hepatitis virus quantity in serum.