Effects of 1,25-(OH)_2D_3 stimulation on expression of ODF and OCIF by human periodontal ligament cells

Objective To clarify the molecular mechanism of periodontal tissue remodeling during orthodontic tooth movement by investigating the effects of 1,25-(OH)2D3 on the expression of ODF and OCIF in human periodontal ligament cells (HPDLCs). Methods HPDLCs were subjected to different doses of 1,25-(OH)2D3 (0,1×10-10,1×10-8,1×10-6 mol/L) for 24 hours in this experiment, mRNA expression of ODF and OCIF were determined by semi quantitative RT-PCR approach. Results The expression of ODF mRNA and increased significantly in a dose-dependent manner,and the expression of OCIF mRNA didn't decrease not significantly. Conclusion 1,25-(OH)2D3 could adjust the proportion of expression of ODF to expression of OCIF. Thus, the bone metabolism was adjusted.