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2002•Journal of Modern StomatologyRequires access

The semi-quantitative RT-PCR study on the expression of OPG and RANKL in human periodontal ligament cells induced by 1alpha,25(OH)_2 vitamin D_3

Xiaotong Li

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Abstract

Objective To study the expression of osteoprotegerin(OPG)and receptor activator nuclear factor kappa B ligand(RANKL)in human periodontal ligament cells(hPDLCs)induced by 1alpha,25(OH) 2 vitamin D 3 (1α,25(OH) 2vitD 3).Methods HPDLCs were cultured by explant method and were induced by 1α,25(OH) 2vitD 3 for 0,2,4,6 days. Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)examination indicated the expression of OPG and RANKL at messenger RNA(mRNA)level in hPDLCs.Results OPG mRNA expression was down-regulated, RANKL mRNA expression was up-regulated, and the ratio of OPG/RANKL was decreased by 1α,25(OH) 2vitD 3 in a time dependent manner.Conclusion The bone metabolism path of OPG and RANKL worked during the process of 1α,25(OH) 2vitD 3 inducing hPDLCs.The conclusion did the groundwork for the study on bone remodeling mechanisms of 1α,25(OH) 2vitD 3.

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Objective To study the expression of osteoprotegerin(OPG)and receptor activator nuclear factor kappa B ligand(RANKL)in human periodontal ligament cells(hPDLCs)induced by 1alpha,25(OH) 2 vitamin D 3 (1α,25(OH) 2vitD 3).Methods HPDLCs were cultured by explant method and were induced by 1α,25(OH) 2vitD 3 for 0,2,4,6 days. Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)examination indicated the expression of OPG and RANKL at messenger RNA(mRNA)level in hPDLCs.Results OPG mRNA expression was down-regulated, RANKL mRNA expression was up-regulated, and the ratio of OPG/RANKL was decreased by 1α,25(OH) 2vitD 3 in a time dependent manner.Conclusion The bone metabolism path of OPG and RANKL worked during the process of 1α,25(OH) 2vitD 3 inducing hPDLCs.The conclusion did the groundwork for the study on bone remodeling mechanisms of 1α,25(OH) 2vitD 3.

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Available abstract

Objective To study the expression of osteoprotegerin(OPG)and receptor activator nuclear factor kappa B ligand(RANKL)in human periodontal ligament cells(hPDLCs)induced by 1alpha,25(OH) 2 vitamin D 3 (1α,25(OH) 2vitD 3).Methods HPDLCs were cultured by explant method and were induced by 1α,25(OH) 2vitD 3 for 0,2,4,6 days. Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)examination indicated the expression of OPG and RANKL at messenger RNA(mRNA)level in hPDLCs.Results OPG mRNA expression was down-regulated, RANKL mRNA expression was up-regulated, and the ratio of OPG/RANKL was decreased by 1α,25(OH) 2vitD 3 in a time dependent manner.Conclusion The bone metabolism path of OPG and RANKL worked during the process of 1α,25(OH) 2vitD 3 inducing hPDLCs.The conclusion did the groundwork for the study on bone remodeling mechanisms of 1α,25(OH) 2vitD 3.

Key concepts: RANKL, Osteoprotegerin, Periodontal fiber, Bone remodeling, Messenger RNA, Chemistry, Real-time polymerase chain reaction, Vitamin D and neurology

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