Correlation Study of Free and Total Ferulic Acid Content in Dried and Fresh Angelica sinensis

Objective:The free and total ferulic acid content in dried and fresh Angelica sinensis Radix was measured by HPLC.Method: Free and total ferulic acid content was determined by HPLC.The separation was performed on a ZORBAX Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm) with acetonitrile-0.085% phosphoric acid(17∶83) as mobile phase at temperature of 35 ℃.The flow rate was 1.0 mL·min-1 and the detected wavelength was set at 316 nm;the injection volume was 10 μL.Result: The linear relationship of free and total ferulic acid was excellent within the range of 0.024 2-0.121 0 μg and 0.024 2-0.192 36 μg,and the recovery rates were 96.34% and 97.35%,respectively.The free ferulic acid in dried A.sinensis Radix of 2009 was the highest and the total ferulic acid in fresh A.sinensis Radix was the highest.Conclusion: This method was simple and accurate with good repeatability and stability.It will help to improve the quality control of A.sinensis Radix.