Study on the Micropropagation of Shoots of Dendranthema morifolium

[Objective]The research aimed to study the influences of different factors on the in vitro propagation of shoots of Dendranthema morifolium.[Method]By using the apical buds and cutting stems with buds of two chrysanthemum species of Xiuqiu and Feizixiao as explants,the effects of different hormones recombination at different concentration on the clone propagation in vitro were studied.[Result]The multiplication rate of adventitious buds from explants of Xiuqiu and Feizixiao can reach 6.8 and 6.4 respectively on the mediums supplemented with BA 1.5 mg/L,IBA 0.5 mg/L and GA 0.1 mg/L.Furthermore,the condition of chrysanthemum shoots root induction had been studied.The results indicated that the best condition of rooting is 1/2MS basal medium supplemented with 0.5 mg/L IBA.[Conclusion]This research could provide scientific and technological basis for the tissue culture and rapid propagation of Xiuqiu and Fei zixiao.