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2004•Chinese Bulletin of BotanyRequires access

Optimization Selection of Genetic Transformation Regeneration System from Leaves of Dendranthema morifolium

Jun Liu, Zhao Lanyong, Feng Zhen, Meirong Zhang, WU Yin-feng

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Abstract

A genetic transfomation regeneration system from aseptic seedling leaves of chrysanthe-mum (Dendranthema morifolium) was developed. Adventitious shoots were directly induced andregenerated from leaf explants of chrysanthemum, and the medium (MS+6-BA 3 mg.L-1+NAA 1 mg.L-1), which could directly induce shoot regeneration, was used. Shoots were rooted on half-strengthMS media with IBA and NAA. The rooted plantlets grew well in greenhouse.

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What this paper is about

A genetic transfomation regeneration system from aseptic seedling leaves of chrysanthe-mum (Dendranthema morifolium) was developed. Adventitious shoots were directly induced andregenerated from leaf explants of chrysanthemum, and the medium (MS+6-BA 3 mg.L-1+NAA 1 mg.L-1), which could directly induce shoot regeneration, was used. Shoots were rooted on half-strengthMS media with IBA and NAA. The rooted plantlets grew well in greenhouse.

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Available abstract

A genetic transfomation regeneration system from aseptic seedling leaves of chrysanthe-mum (Dendranthema morifolium) was developed. Adventitious shoots were directly induced andregenerated from leaf explants of chrysanthemum, and the medium (MS+6-BA 3 mg.L-1+NAA 1 mg.L-1), which could directly induce shoot regeneration, was used. Shoots were rooted on half-strengthMS media with IBA and NAA. The rooted plantlets grew well in greenhouse.

Key concepts: Shoot, Regeneration (biology), Explant culture, Biology, Botany, Chrysanthemum morifolium, Seedling, Horticulture

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