Distribution and drug resistance analysis of Escherichia coli and Klebsiella pneumoniae producing extended spectrum beta-lactamases

Objective To investigate the distribution and drug resistance of Escherichia coli and Klebsiella pneumoniae producing extended spectrum beta-lactamases(ESBLs) in hospital.Methods ESBLs was detected by CLSI/NCCLS phenotypic confirmatory test in E.coli(n=307) and K.pneumoniae(n=202) which were isolated from July 2004 to July 2006 in the hospital.Antimicrobial susceptibility was tested by Kirby-Bauer method.The distribution and drug resistance of strains producing ESBLs was analyzed.Results The detection rate of E.coli and K.pneumoniae producing ESBLs was 38.4%(118/307) and 31.7%(64/202),respectively.The distribution of specimens mainly came from urine,sputum and respiratory secretions.The distribution of wards mainly came from pulmonary disease,neurosurgery,infectious,urology and ICU ward.The strains producing ESBLs were very susceptible to imipenem and meropenem,and relatively susceptible to piperacillin/tazobactam,cefoperazone/sulbactam and cefoxitin.The resistance rate of the strains producing ESBLs was high to other antimicrobial.Conclusion The E.coli and K.pneumoniae strains producing ESBLs distribute various kinds of specimens from different wards.Imipenem and meropenem of carbapenems could be better antimicrobial to treat the infection by strains producing ESBLs.