2009•Suzhou Daxue xuebao. Yixue banRequires access

Effects of Ursolic Acid on Proliferation and Apoptosis of SK-BR-3 Cells

Zhou Wen-xuan

Abstract

Objective To investigate the inhibitory and apoptotic effect of ursolic acid(UA) on the proliferation of human breast cancer SK-BR-3 cells.Methods The effect of UA on the proliferation of SK-BR-3 cells was measured by MTT method.Hoechst 33258 fluorescent dying and electron microscope were used to observe UA induced morphological changes.The cell cycle was observed with flow cytometry.DNA fragmentation was observed by DNA agarose gel electrophoresis.Results UA inhibitied the growth of SK-BR-3 cells in a dose-and time-dependent manner.The morphological changes of apoptosis,such as nuclear fragmentation or condensed particles were identified after incubation with UA.Cell cycle analysis revealed that SK-BR-3 cells treated with UA for 48 h were blocked predominantly in G0/G1 phase(P0.05).Large fragments of DNA were observed by DNA agarose gel electrophoresis.Conclusion UA has the effects of inhibition of SK-BR-3 cell proliferation and induction of cell apoptosis.

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Key concepts: Agarose gel electrophoresis, Apoptosis, Ursolic acid, DNA fragmentation, Molecular biology, Cell cycle, Flow cytometry, Fragmentation (computing)