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2006•Xiandai linchuang yixue shengwu gongchengxue zazhiRequires access

Proliferation Inhibition and Apoptosis Induction of U937 Cells by Quercetin

Huimin Lu

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Abstract

Objective To investigate the effect of quercetin(Que) on U937 cells,a kind of human monocyte leukemia cells.Methods Inhibition rate of quercetin on U937 was assayed by MTT method,cell apoptosis by fluorescence microscope,flow cytometry(FCM) and agarose gel electrophoresis.Results Proliferation of U937 cells were significantly inhibited by quercetin.There were dose-effect relation and time-effect relation.Morphologic changes of apoptotic U937 cells induced by quercetin detected with fluorescence microscope.The ratio of apoptotic and necrosic cells increased accompany with added quercetin concentration.DNA agarose gel electrophoresis showed the typical DNA ladder of apoptosis.Cell cycle was specificly blocked in S phase.Apoptosis cusp was revealed by FCM.Conclusions Quercetin inhibits proliferation of U937 cells,induces cell cycle specificly apoptosis.

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What this paper is about

Objective To investigate the effect of quercetin(Que) on U937 cells,a kind of human monocyte leukemia cells.Methods Inhibition rate of quercetin on U937 was assayed by MTT method,cell apoptosis by fluorescence microscope,flow cytometry(FCM) and agarose gel electrophoresis.Results Proliferation of U937 cells were significantly inhibited by quercetin.There were dose-effect relation and time-effect relation.Morphologic changes of apoptotic U937 cells induced by quercetin detected with fluorescence microscope.The ratio of apoptotic and necrosic cells increased accompany with added quercetin concentration.DNA agarose gel electrophoresis showed the typical DNA ladder of apoptosis.Cell cycle was specificly blocked in S phase.Apoptosis cusp was revealed by FCM.Conclusions Quercetin inhibits proliferation of U937 cells,induces cell cycle specificly apoptosis.

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Available abstract

Objective To investigate the effect of quercetin(Que) on U937 cells,a kind of human monocyte leukemia cells.Methods Inhibition rate of quercetin on U937 was assayed by MTT method,cell apoptosis by fluorescence microscope,flow cytometry(FCM) and agarose gel electrophoresis.Results Proliferation of U937 cells were significantly inhibited by quercetin.There were dose-effect relation and time-effect relation.Morphologic changes of apoptotic U937 cells induced by quercetin detected with fluorescence microscope.The ratio of apoptotic and necrosic cells increased accompany with added quercetin concentration.DNA agarose gel electrophoresis showed the typical DNA ladder of apoptosis.Cell cycle was specificly blocked in S phase.Apoptosis cusp was revealed by FCM.Conclusions Quercetin inhibits proliferation of U937 cells,induces cell cycle specificly apoptosis.

Key concepts: U937 cell, Apoptosis, Agarose gel electrophoresis, Cell cycle, Quercetin, Flow cytometry, Molecular biology, Cell growth

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