Apoptosis of SMMC-7721 cells induced by ursolic acid

Objective:To observe the inhibition and apoptosis effect of ursolic acid on SMMC-7721 cells.Methods:MTT-based cytotoxicity assay was used to test the inhibition effect of different concentration(such as 5,10,20,30,40,50 μmol / L)UA on SMMC-7721 cells;Acridine orange(AO) staining,electron microscopy and flow cytometry were used to detected cell apoptosis.Results:UA significantly inhibited SMMC-7721 cells proliferation in a dose-dependent manner.AO staining was observed SMMC-7721 cells for 35.2μmol/L UA role after 48 hours,cells were seen under fluorescent microscope shrinkage,nuclear fragmentation,chromatin morphological changes of apoptosis;SMMC-7721 cells showed significant apoptosis morphological changes,nuclear chromatin margination and the accumulation occurs,intracellular part of the mitochondrial swelling under electron microscope;Apoptosis rate of SMMC-7721 cells was(67.91 ± 5.24)%,and the control group was(2.95 ± 0.56)%,the difference was statistically significant(P 0.05).Conclusion:UA can inhibited the proliferation of SMMC-7721 cells by induced apoptosis.