2002•Xibei nongye xuebaoRequires access
In vitro plant regeneration from cotyledon explant of cotton (Cossypium h irsutum)
Hai Zhang, Yi Yonghua, Gao Xiao-hua, Xing Hongyi, Jia Tao
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Abstract
Effects of hormones on differentiation of adventitious buds and plant regeneration were studied in cotyledon culture of cotton in vitro, BA was considered as the best growth regulator for adventitious bud differentiation within 4 cytokinins. The combination of IAA and BA raised the differentiation frequency obviously. The higher differentiation frequency of adventitious buds was obtained in the medium MS+4.0 mg/L BA+0.5 mg/L IAA, while in the media with NAA and BA, the differentiation frequency of adventitious buds reduced. However, 2,4 D had the inhibition effect on the bud differentiation GA 3 can promote the elongation of adventitious buds. The elongated shoots rooted in media MS without hormore or MS+0.5 mg/L IAA; and developed into the complete plant.