Inhibitory effect of different preparations of antineoplastic polypeptide from Buthus martensii venom on hepatoma 22 and influence of APBMV on the immune function of tumor bearing mice

Aim: To observe the influence of antineoplatic polypeptide from Buthus martensii venom (APBMV) on the growth of hepatoma 22(H 22 )and the immunity of H 22 bearing mice. Methods: ①The 45 mice inoculated by H 22 cells for 24 hours were divided into 4 groups:non normal control group,5 Fu treated group,APBMV injection and capsule groups. H 22 bearing mice were treated by different chemicals for 10 days and the weight changes of H 22 tumor and spleen were observed. The tumor inhibitory rate and spleen weight index were calculated. ②The 40 mice inoculated by H 22 cells for 24 hours were divided into 4 groups:non normal control group,5 Fu treated group,APBMV injection or/and 5 Fu groups. H 22 bearing mice were treated with APBMV injection or/and 5 Fu for 5 days. The activity of NK cell and the transformation rate of lymphocytes were observed. Results: ①Inhibitory rates in 5 Fu and APBMV injection groups were 47.81% and 40.07%, respectively. Compared with the non normal control group, the differences were significant ( P 1.05). The spleen weight indexes were 70.02 mg/10 g and 71.22 mg/10 g in APBMV injection and capsule groups, respectively. Compared with the non normal control group, the differences were significant ( P 0.01). ②The activity of NK cell was enhanced remarkably and the transforming rate of lymphocytes was increased evidently in both APBMV injection group and APBMV injection+5 Fu group ( P 0.05). Conclusion: APBMV could inhibit the growth of H 22 and strengthen the immunity function of H 22 brearing mice.