2009•Zhonghua zhongyiyao xuekanRequires access
Effect of Yiqiyangyin and Huoxuehuayu Methods on the Expression of TGF-β_1 in Rats with Pulmouary Fibrosis
Xu Li
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Abstract
Objective:To observe the effect on the rat models of pulmonary fibrosis by Danshen and Shenmai parenteral solution with the pulmonary pathological changes,expression of TGF-β1 in pulmonary tissue and bronchoalveolar lavage fluid(BALF).And discuss the action mechanism of yiqiyangyin and huoxuehuayu methods in the pulmonary fibrosis.Methods:Reproduce the rat models of pulmonary fibrosis by injecting bleomycin to trachea,72 SD rats were randomly divided into four groups,including the normal group,the modeled group,the Danshen group,the Danshen and Shenmai group.Then treat the latter three rat models with intraperitoneal injection by physiological saline,Danshen parenteral solution,Danshen and Shenmai parenteral solution meanwhile.The rats in each group were partially killed in 7th,14th,28th day,the BALF was selected and the pulmonary tissue was incised to make pathological sections which were stained with HE and were stained with TGF-β1 immunohistochemical techniqu.Using computer image analysis system evaluate the result of the pulmonary pathological changes of each group on the 28th day.Also the expression of TGF-β1 in pulmonary tissue was analyzed with comptuer image system and the concentration of TGF-β1-protein in BALF was detected by way of ELISA on 7th,14th,28th day.Results: Treated with Danshen parenteral solution,Danshen and Shenmai parenteral solution,the pulmonary fibrosis and pulmonary alveolitis of rat models could be alleviated,and the latter group could be significantly decreased(P0.05).At the same time,we also observed that in the former two groups the expression of TGF-β1 in pulmonary tissue and BALF was lower than the modeled group(P0.01),and on the 14th day the Danshen and Shenmai group was lower than the Danshen group(P0.05).Conclusion:The yiqiyangyin and huoxuehuayu methods which were represented by Danshen and Shenmai have good effect on relieving the models of pulmonary fibrosis,and its action mechanism is connected to decreasing the expression of TGF-β1.