2012•China Medical HeraldRequires access
Determination of Paeoniflori in Taohongcuyun Mixture by HPLC
Zhu Mingwei
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Abstract
Objective To establish a standard for determination of Paeoniflori in Taohongcuyun Mixture.Methods Chromatographic colum was Agilent-C18,the mobile phase was acetonitrile-0.1% phosphoric acid solution(14∶86),the flow rate was 1.0 mL/min,the detection wavelength was 230 nm.Results The linear range of Paeoniflori was 0.308 2-6.164 0 μg,r = 0.999 9.The average recovery of Paeoniflori was 98.79% and RSD was 0.96%.Conclusion The method is simple,sensitive and accurte,and can be used to determine Paeoniflori in Taohongcuyun Mixture and quality control of Taohongcuyun Mixture.