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2006•Qilu Pharmaceutical AffairsRequires access

Determination of archin in Jiannaoning Mixture by HPLC

Wang Deng-xu

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Abstract

OBJECTIVE To establish HPLC method for determination of archin in Jiannaoning Mixture.METHODS The Kromasil C_(18)(250mm×4 6mm,5μm) column was used.The column temperature was at 25℃.The mobile phase was acetonitrile-water-acetic acid(60∶40∶1).The detection wavelength was at 437nm.RESULTS The standard curve was linear in the concentration range 0.0969～0.226μg.The average recovery was 98.10%,RSD=1.05%(n=6).CONCLUSION The method is convenient,sensitive and accurate.It can be used for quality control of Jiannaoning Mixture.

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What this paper is about

OBJECTIVE To establish HPLC method for determination of archin in Jiannaoning Mixture.METHODS The Kromasil C_(18)(250mm×4 6mm,5μm) column was used.The column temperature was at 25℃.The mobile phase was acetonitrile-water-acetic acid(60∶40∶1).The detection wavelength was at 437nm.RESULTS The standard curve was linear in the concentration range 0.0969～0.226μg.The average recovery was 98.10%,RSD=1.05%(n=6).CONCLUSION The method is convenient,sensitive and accurate.It can be used for quality control of Jiannaoning Mixture.

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Available abstract

OBJECTIVE To establish HPLC method for determination of archin in Jiannaoning Mixture.METHODS The Kromasil C_(18)(250mm×4 6mm,5μm) column was used.The column temperature was at 25℃.The mobile phase was acetonitrile-water-acetic acid(60∶40∶1).The detection wavelength was at 437nm.RESULTS The standard curve was linear in the concentration range 0.0969～0.226μg.The average recovery was 98.10%,RSD=1.05%(n=6).CONCLUSION The method is convenient,sensitive and accurate.It can be used for quality control of Jiannaoning Mixture.

Key concepts: Chromatography, High-performance liquid chromatography, Acetonitrile, Acetic acid, Calibration curve, Chemistry, Linear range, Standard curve

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