2002•Chinese Journal of DermatologyRequires access
Strain Typing by PCR in Trichophyton rubrum
Min Li, Jiajun Wang, Qiangqiang Zhang, Li Li, Hekui Lan
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Abstract
Objective
To develop a rapid and reproducible m ethod in strain identification for T.rubrum.
Methods
Two novel tandem repeat subelements(TRSS),trs-1 and trs-2,located in the T.rubrum rDNA non-transcribed spacer(NTS) were amplified from 20 strains of T.rubrum.The PCR products were cloned into seq uencing vector PGEM-T for sequencing.
Results
Specific amplification of trs-1 and trs-2 produced strain-characteristic band patterns(PCR types),the sequence from trs-1 and trs-2 also supported this finding.The trs-1 sequence variation was found in a strain of T.rubrum which can grow at 37℃.
Conclusion
The characteristic fingerprints generated by this PCR assay provide a rapid,stable molecular typing method to study the epidemiology and pathogenicity of T.rubrum infection.
Key words:
Trichophyton; Mycological typing techniques