2001•Europe PMC (PubMed Central)Requires access
PCR fingerprint identification of Trichophyton rubrum and Trichophyton mentagrophytes using single primers specific to minisatellites and simple repetitive DNA sequence
Hong Zhu, Wanqing Liao, Jianxin Dai, Zhigang Li, Jianhua Wu, Wen Hai
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Abstract
Objective: To observe the difference between the species Trichophyton rubrum and Trichophyton mentagrophytes. Methods: Three primers, including (GACA) 4, (GTG) 5 and M13 core sequence (5′ GAGGGTGGCGGTTCT 3′), were used to distinguish variations among 23 clinical isolates of T. rubrum and 11 of T. mentagrophytes. Results: Different PCR fingerprinting were seen between T. rubrum and T. mentagrophytes by using 3 different primers, especially amplification with primer (GACA) 4 could give more distinct bands. Conclusion: T. rubrum and T. mentagrophytes can be distinguished by PCR, (GACA) 4 is the most suitable primer. [