Analysis of Organic Acids in Grape by Reversed Phase High Performance Liquid Chromatography

A quantitative reversed-phase high performance liquid chromatography method using diode array detector has been developed for the determination of tartaric acid,fumarate citric acid and malic acid in grape.A Hibar column was used with phosphate buffer solution(ph=2.5)as the mobile phase at a flow rate of 1.0mL/min.The column temperature was 30℃ and the UV detection wavelength was 214 nm.The calibration curve for citric acid and malic acid was in good linearity in the range of 15.0-63.4μg with the regression equation Y=1064.5X+3.58,r=0.9952,and 10-46.8μg,Y=1066.3X-4.31,r=0.9997,respectively.The average recoveries of three concentration levels for citric acid and malic acid in grape were 95.5%-96.5%and 100.2%-103.8%,respectively.RSD were 0.11%-0.38%and 0.41%-2.53%,respectively.The detection limits for citric acid and malic acid were 0.2μg and 0.1μg(S/N=3),respectively.The results indicate that this method is fast,sensitive,reproducible and practical for the routine analysis of organic acids in grape.