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Determination of Paeoniflorin Content in Naoxueshuan Tablet

Xiaoyu Zhang

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Abstract

Objective:To establish a HPLC method for determination of paeoniflorin content in Naoxueshuan tablet.Methods:HPLC was used with the Diamonsil C18 column(4.6 mm×150 mm,5μm),Acetonitrile-water(14∶86) as mobile phase,flow rate 1.0 mL/min.The detective wave-length was 230 nm.Results:It had a good linearity within 0.2432～1.2160μg for paeoniflorin.The average recoveries was 98.6%(n=5) and relative standard deviation(RSD) was 1.8%.Conclusion:This method is specific with accuate and reliable results,which can be used to control the quality of the products effectively.

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What this paper is about

Objective:To establish a HPLC method for determination of paeoniflorin content in Naoxueshuan tablet.Methods:HPLC was used with the Diamonsil C18 column(4.6 mm×150 mm,5μm),Acetonitrile-water(14∶86) as mobile phase,flow rate 1.0 mL/min.The detective wave-length was 230 nm.Results:It had a good linearity within 0.2432～1.2160μg for paeoniflorin.The average recoveries was 98.6%(n=5) and relative standard deviation(RSD) was 1.8%.Conclusion:This method is specific with accuate and reliable results,which can be used to control the quality of the products effectively.

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Available abstract

Objective:To establish a HPLC method for determination of paeoniflorin content in Naoxueshuan tablet.Methods:HPLC was used with the Diamonsil C18 column(4.6 mm×150 mm,5μm),Acetonitrile-water(14∶86) as mobile phase,flow rate 1.0 mL/min.The detective wave-length was 230 nm.Results:It had a good linearity within 0.2432～1.2160μg for paeoniflorin.The average recoveries was 98.6%(n=5) and relative standard deviation(RSD) was 1.8%.Conclusion:This method is specific with accuate and reliable results,which can be used to control the quality of the products effectively.

Key concepts: Paeoniflorin, Medicine, Chromatography, High-performance liquid chromatography, Relative standard deviation, Content determination, Chemistry, Detection limit

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