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2009•Chinese Journal of Modern Drug ApplicationRequires access

Determination of Paeoniflorin in Huayuquban Tablets With HPLC Method

Ruwei Wang

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Abstract

Objective to establish HPLC method for determination of Paeoniflorin in Huayuquban Tablets.Methods The chromatographic conditions consisted of Diamonsil C18 column(4.6 mm×150 mm,5 μm)with the mobile phase of acetonitrile-water-chemical reagent phosphoric acid(20:80:0.1)at the flow rate of 1.0 ml/min,the deter-mination wavelength was 230 nm and sample size was 10 μl.Results Paeoniflorin showed a good linearity at the range of 0.3964～3.5676 mg/mL,r=0.9999,average recovery was 99.2%,RSD%= 1.51%(n=6).Conclusion The method is simple,accurate,high sensitive and good reproducibility,can be used as the method for determination of Paeoniflorin in Huayuquban Tablets.

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What this paper is about

Objective to establish HPLC method for determination of Paeoniflorin in Huayuquban Tablets.Methods The chromatographic conditions consisted of Diamonsil C18 column(4.6 mm×150 mm,5 μm)with the mobile phase of acetonitrile-water-chemical reagent phosphoric acid(20:80:0.1)at the flow rate of 1.0 ml/min,the deter-mination wavelength was 230 nm and sample size was 10 μl.Results Paeoniflorin showed a good linearity at the range of 0.3964～3.5676 mg/mL,r=0.9999,average recovery was 99.2%,RSD%= 1.51%(n=6).Conclusion The method is simple,accurate,high sensitive and good reproducibility,can be used as the method for determination of Paeoniflorin in Huayuquban Tablets.

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Available abstract

Objective to establish HPLC method for determination of Paeoniflorin in Huayuquban Tablets.Methods The chromatographic conditions consisted of Diamonsil C18 column(4.6 mm×150 mm,5 μm)with the mobile phase of acetonitrile-water-chemical reagent phosphoric acid(20:80:0.1)at the flow rate of 1.0 ml/min,the deter-mination wavelength was 230 nm and sample size was 10 μl.Results Paeoniflorin showed a good linearity at the range of 0.3964～3.5676 mg/mL,r=0.9999,average recovery was 99.2%,RSD%= 1.51%(n=6).Conclusion The method is simple,accurate,high sensitive and good reproducibility,can be used as the method for determination of Paeoniflorin in Huayuquban Tablets.

Key concepts: Paeoniflorin, Chromatography, Chemistry, Phosphoric acid, High-performance liquid chromatography, Reagent, Reproducibility, Organic chemistry

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