Effect of short-term all-trans retinoic acid on cell proliferation and apoptosis of human colorectal cancer LoVo cell

Objective To investigate the effect of short-term all-trans retinoic acid(ATRA) on cell proliferation and apoptosis of human colorectal cancer LoVo cell.Methods The growth inhibitory rate of ATRA on LoVo cell was detected by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide(MTT) and the suitable drug concentration of ATRA was defined.Cell cycle and apoptosis induced by ATRA were assessed by flow cytometry.Results 1.0 μmol·L-1ATRA was chosen as work concentration for further experiment.The cells in G1 phase began to increase after 1.0 μmol·L-1ATRA treatment for 12 hours,and the cells in G1 phase were significance increase,while the cells in S and G2/M phase decreased after treatment for 48 hours(P0.05).Compared with control group,there were statistical significance in early and total apoptosis rates of LoVo cell induced by 1.0 μmol·L-1 ATRA treatment for 48 and 72 hours groups(P0.01);there was statistical significance in early and total apoptosis rates of LoVo cell induced by ATRA between treatment for 48 and 72 hours groups(P0.05).Conclusion A short-term 1.0 μmol·L-1 ATRA can significantly inhibit LoVo cell growth by blocking the cells into G1 phase and inducing the cells early apoptosis.