Research on the comparability of α-fetoprotein result measured with different kinds of assay system

Objective To study the comparability of α-fetoprotein(AFP) results obtained with different detection system. Methods 4 kinds of chemiluminescence immunoassay system(Bayer ACS 180,Bayer CENTAUR,Johnson VITROS ECI and DPC immulite-1000) were used to detect AFP concentration in 2 kinds of quality control specimen(Bayer and DPC) and 31 patient samples of different concentration.The results were analysed with statistics methods. Results Analysis of variance showed that AFP results from different controls and patient samples had significant difference in different assay system(P0.01).The correlation coefficient of each detection system was above 0.975 and the reliability coefficient of them was closed to 1.When Johnson VITROS ECI was regarded as a target detection system in order to evaluate the acceptability of other detection system,all detection systems in our study could be accepted by clinic assessment. Conclusions The precision of AFP results in the 4 kinds of detection system accord to clinical requirement.Comparability exists in the evaluation of clinical acceptability.