The Establishment of Diabetes Atherosclerosis Rat Model

Objective To establish rat model of type 2 diabetes atherosclerosis.Methods Forty male SD rats(6 weeks old) were randomly divided into two groups:control group(n=20) and model group(n=20).The control group was fed with normal diet and injected with citrate buffer.After 4 weeks of normal diet,model group was given streptozotocin(STZ)(35 mg/kg) consecutively by intraperitoneal injection for 3 days,and then fed with high-fat and high-sugar diet for 8 weeks.Blood glucose,total cholesterol(TC),triglyceride(TG),high density lipoprotein(HDL) and low density lipoprotein(LDL) were measured.The rats were killed to observe pathological changes of aorta.Results Blood glucose of model group on the 3rd,10th day after consecutive STZ injection was significantly higher than that of the control group(P0.01).After the rats were fed with high-sugar and high-fat diet for 8 weeks,serum lipids of model group was significantly higher than that of the control group(P0.01).Aorta hypertrophy could be observed by the naked eye.The early pathological changes of type 2 diabetes atherosclerosis,such as fibroplastic proliferation and increase of nucleated cells,could be seen under the microscope.Conclusion The rat model of type 2 diabetes atherosclerosis could be established successfully by consecutive injection of low dose streptozotocin together with high-sugar and high-fat diet.