ESTABLISHMENT OF TYPE 2 DIABETES MOUSE MODEL BY HIGH FAT DIET AND DIFFERENT DOSE STREPTOZOTOCIN

Objective:To investigate the effects of establishing type 2 diabetes mouse model by using different dose streptozotocin(STZ) combined high fat and sugar diet.Methods:40 Kunming mice were randomly divided into the following 4 groups with 10 mice in each group: normal control group and model group A,B,C.The mice in normal control group were fed with basal diet.The mice in 3 model groups were fed with high fat and sugar diet for 4 weeks,then the mice in model group A,B,C were peritoneally injected STZ in different dose(60,80,100mg/kg);then the mice in 3 model groups were fed with high fat and sugar diet for further 3 weeks.The blood glucose and insulin level in serum were respectively detected before and after establishing type 2 diabetes model.Results:The blood glucose of mice in 3 model groups were all obviously higher than that in normal control group(P0.01,P0.05).Moreover,the blood glucose of mice in model group C were obviously higher than that in model group A and B(P0.01).There had no obvious differences about insulin level of rats in these 4 groups(P0.05).Conclusions:High fat and sugar diet combined STZ can be used to make type 2 diabetes mice model,which has similar phenotypic characteristics and pathogenesis process of human.