Determination of total flavanoid in Semen cuscutae by spectrofluorimetry

Quercetin was used as standard material to establish a new method for determining total flavanoid in Semen cuscutae.The results showed that the detectioning limit was 2.51×10-7 g/L,the range of linearity with concentration of Quercetin was between 6.7×10-6~6×10-4 g/L,linearity relationship was y=531.84048x-3.54448,R2=0.99903.The average amount of total flavanoid in Semen cuscutae was 0.4727 mg/g,RSD was 1.9514%,the extracting rate of the flavones was 89.24%.It is simple and accurate method to determine total flavanoid in Semen cuscutae with spectrofluorophotometer.The new method can be used for quality control of chinese medicinal plant Semen cuscutae.