Determination of Total Flavanoid in Polygonum Aviculare by Spectrofluorimetry

Objective:To establish a new method for determining total flavanoid in Polygonum aviculare.Methods:Rutin was used as standard material to determine fluorescence with spectrofluorophotometer and draw standard curve.Total flavanoid was extracted with 60% ethanol by continuous thermal reflux.The fluorescence of it was determined in the same condition,and the content was ob- tained on the basis of regression equation.Results:When the rutin concentration ranged from 0μg/mL to 22μg/mL,fluorescence and rutin concentration showed the good linear relationship.Linearity relationship was Y=-8.973X+22.581,and the correlative coefficient was 0.9771.The average amount of total flavanoid in Polygonum aviculare was 17.05μg/mL,and the extracting rate of the flavones was 6.82 %.Conclusions:It is simple,rapid,and accurate method to determine total flavanoid in Polygonum aviculare with spectrofluoropho- tometer,and the method was suggested to be adopted.