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2013•Basic & Clinical MedicineRequires access

Loop-mediated isothermal amplification technology and its application in pathogenic microorganism detection

Wenli Ma

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Abstract

Loop-mediated isothermal amplification(LAMP) technology is a novel nucleic acid isothermal amplification technology developed in recent years.Because of its high effiency,rapidity,specificity,and simplicity,scientists believe it will be a routine amplification technology replacing the conventional PCR method.This paper briefly reviews the principle of the technology,techinical characteristics,progress,and its application in pathogenic microorganism detection.

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What this paper is about

Loop-mediated isothermal amplification(LAMP) technology is a novel nucleic acid isothermal amplification technology developed in recent years.Because of its high effiency,rapidity,specificity,and simplicity,scientists believe it will be a routine amplification technology replacing the conventional PCR method.This paper briefly reviews the principle of the technology,techinical characteristics,progress,and its application in pathogenic microorganism detection.

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Available abstract

Loop-mediated isothermal amplification(LAMP) technology is a novel nucleic acid isothermal amplification technology developed in recent years.Because of its high effiency,rapidity,specificity,and simplicity,scientists believe it will be a routine amplification technology replacing the conventional PCR method.This paper briefly reviews the principle of the technology,techinical characteristics,progress,and its application in pathogenic microorganism detection.

Key concepts: Loop-mediated isothermal amplification, Isothermal process, Nucleic acid, Microorganism, Biochemical engineering, Nanotechnology, Biology, Materials science

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