Screening of biocontrol bacteria against sesame Fusarium Wilt

The study isolated 421 endophytic bacteria from 36 samples of healthy sesame roots growing in eastern Henan province.These bacterial strains were isolated on King's B medium(KBM), incubated on King's B medium(KBM), potato dextrose agar(PDA) and malt yeast glucose agar(MYG). Antagonistic activity of these bacteria against Fusarium oxysporium was tested by dural culture method.The result showed that 3 bacterial strains(A21, G20, G36) had significant antagonistic activity, and the biological control efficacies were52%, 40%, 67% respectively. The detection of the enzyme production ability indicated that the 3 bacterial strains produced different cell wall degrading enzymes, and possibly had different bio-control mechanism. The test of physiological and biochemical characteristics of endophytic bacteria indicated that G36 belonged to Bacillus sp.