Cultivation and Identification of Mouse′ Aorta Vascular Endothelial Cells and Proliferation by Macrophage Migration Inhibitory Factor

Objective To explore cultivation and identification of mouse′ vascular endothelial cells(VEC) and accommodation on proliferation by macrophage migration inhibitory factor(MIF).Methods Murine aorta VEC were got rid of periphery fat and connective tissue,cut arterial circle to 0.5 mm of circularity and put into 12 apertures of cultural laminae treated with matrigel.The trypsin digestion method was used after topful cells.The single cell suspension was purified by anti-CD146 immunomagnetic beads.The cells purity was detected with anti-CD31 antibody.Cells were processed by different concentrations of MIF or MIF inhibitor when cells inosculated at 70%-80% range,after cultivated by 104 cells/apertures added into 96 apertures of cultural laminae.The proliferation of the pure VECs populations was detected by methyl thiazolyl tetrazolium(MTT) after 48 h.Results The purity of VEC was 95% after purified by CD146 magnetic beads.Low dose of MIF could promote VECs proliferation significantly which proved by detected results of MTT and had dose-effect relationships,but high dose of MIF had the same role with MIF inhibitor which could restrain VECs proliferation.Conclusion The VEC is isolated successfully by CD146 magnetic beads with high purity.A certain dosage range of MIF can stimulate vascular endothelial cell proliferation.The MIF may play an important role in the vascular pathophysiological reaction.