Inhibition Effect of the Recombinant Soluble Vascular Endothelial Growth Inhibitor on the Proliferation of Vascular Endothelial Cells in vitro

Objective To test the recombinant soluble vascular endothelial growth inhibitor(\{VEGI\}) for its inhibition activity against the proliferation of new\|born bovine aortic endothelial cells(BAEC), human umbilical vein endothelial cell line ECV304 and mouse pulmonary capillary endothelial cells(MPCEC), B\|16 melanoma cells and L\|929 fibroblast in vitro. Methods The effects of recombinant VEGI on the proliferation of vascular endothelial cells and tumor cells were assayed by MTT technique in vitro. Results The recombinant VEGI inhibited the proliferation of BAEC, ECV304 and MPCEC in vitro in a dose\|dependent manner. In contrast, recombinant VEGI had no influence on the growth of B\|16 and L\|929 cells under the same culture conditions. Conclusion The recombinant soluble VEGI could specifically inhibit the proliferation of endothelial cells but could not inhibit the proliferation of tumor cells in vitro. This data suggest that VEGI might be highly valuable to angiogenesis inhibition based cancer therapy.