[Effects of different doses of nano silver on vascular endothelial cell proliferation in vitro].

AIM: To explore the effects of different doses of nano silver of proliferation of cultured vascular endothelial cells in vitro. METHODS: The hearts of three newborn SD rats 5 day old were mechanically minced the enzymatically digested with collagenase and trypsin, then vascular endothelial cell were counted, washed and resuspended in Dulbecco's minimumes sential medium (DMEM) added with 20% heat in activated fetal calf serum, then inoculated d in 2% gelatin coated tissue culture flasks. Vascular endothelial cells at passage 3 were used in the experiment. Except for the normal control group, the vascular endothelial cells were cultured with nano silver in various concentrations (0.5, 0.25, 0.125, 0.0625, 0.03125 g/l) for 24 hours, and the morphology and the number of the cultured endothelial cells were observed. Methly thiazolyl tetrazolium (MTT) colorimetry was used to determine the proliferation of the cultured vascular endothelial cells. Flow cytometry (FCM) were used to detect the proliferation index (PI) of the vascular endothelial cells and the expressions of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemical method. RESULTS: The cell morphology was normal under the inverted microscoped in each group. The number and proliferation activities of vascular endothelial cells were significantly decreased by 0.5, 0.25, 0.125, 0.0625, 0.03125 g/l nano silver compared with those of the blank control group, especially the 0.25 g/l nao silver group, and there were no remarkable changes in with 0.5 g/L, 0.125 g/L, 0.0625 g/L and 0.03125 g/L nano silver groups compared to each other. The same results were seen in the positive rate of PCNA expression and PI. CONCLUSION: Nano silver has dose-dependent effects on the proliferation activity of vascular endothelial cells. It inhibited the proliferation of vascular endothelial cells.