Comparative study on anther and microspore-isolated cultures in Chinese cabbage

This paper compared the methods of anther and microspore-isolated cultures in Chinese cabbage.The results showed that the difference of genotype between varieties was the key factor affecting production of the pollen embryo.The embryo inducement efficiency of microspore-isolated culture was 10 times greater than that of pollen culture.But the regeneration rate of pollen plants of microspore-isolated culture was much lower than that of pollen culture.Many key techniques of microspore-isolated culture should be improved in order to widely apply to breeding work.