Isolated microspore culture and ploidy identification of microspore-derived plants in Brassica campestris ssp.chinensis

Several factors affecting isolated microspore culture of non-heading Chinese cabbage were studied.The results showed that the genotype was the most important factor on the inducing rate of microspore-derived embryos,and no significant difference was observed between reciprocal cross.The best inflorescence age of the donor plants was 6-10 d after bloom.Higher agar concentration from 8 g·L-1 to 12 g·L-1 could significantly promote the regeneration of embryos.Based on the flow cytometry and identification of the morphological characteristics,a high level of the spontaneous doubling efficiency from microspore-derived plants was observed in non-heading Chinese cabbage,even to 90%,and there were obvious differences between genotypes.