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Determination of Kojic Acid in Food by LC-MS-MS

Quan Wen, Kong Jia, GU Bei-li

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Abstract

A quantitive assay for the determination of Kojic acid(KA) content in food was established using LC-MS-MS.The samples were separated on a ZOBAX-XDB C18 column using methanol-Na2HPO4(0.005 mol/L) as mobile phase(5:95).The linear range of the method was 1.00～100 μg/mL and the average recovery was up 85%.The ESI mass spectrometry ion source,solvent-removing temperature,ionization voltage,solvent removal gas flow,cone gas flow,monitoring mode,quantitative ion,and collision energy were the positive ionization source,350 ℃,25 V,500 L/h,50 L/h,multiple reaction monitoring(multiplereaction monitoring,MRM) mode,142.8/68.2,20v,142.8/96.4,and 15 V,respectively.

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What this paper is about

A quantitive assay for the determination of Kojic acid(KA) content in food was established using LC-MS-MS.The samples were separated on a ZOBAX-XDB C18 column using methanol-Na2HPO4(0.005 mol/L) as mobile phase(5:95).The linear range of the method was 1.00～100 μg/mL and the average recovery was up 85%.The ESI mass spectrometry ion source,solvent-removing temperature,ionization voltage,solvent removal gas flow,cone gas flow,monitoring mode,quantitative ion,and collision energy were the positive ionization source,350 ℃,25 V,500 L/h,50 L/h,multiple reaction monitoring(multiplereaction monitoring,MRM) mode,142.8/68.2,20v,142.8/96.4,and 15 V,respectively.

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Available abstract

A quantitive assay for the determination of Kojic acid(KA) content in food was established using LC-MS-MS.The samples were separated on a ZOBAX-XDB C18 column using methanol-Na2HPO4(0.005 mol/L) as mobile phase(5:95).The linear range of the method was 1.00～100 μg/mL and the average recovery was up 85%.The ESI mass spectrometry ion source,solvent-removing temperature,ionization voltage,solvent removal gas flow,cone gas flow,monitoring mode,quantitative ion,and collision energy were the positive ionization source,350 ℃,25 V,500 L/h,50 L/h,multiple reaction monitoring(multiplereaction monitoring,MRM) mode,142.8/68.2,20v,142.8/96.4,and 15 V,respectively.

Key concepts: Chemistry, Chromatography, Kojic acid, Solvent, Mass spectrometry, Selected reaction monitoring, Analytical Chemistry (journal), Electrospray ionization

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