Determination of Perchlorate in Surface Water by High Performance Liquid Chromatography-Electrospray Ion Source-Tandem Mass Spectrometry

A method was developed for determination of perchlorate in surface water based on high performance liquid chromatography-electrospray ion sourcetandem mass spectrometry( HPLC-ESI-MS / MS). The anion exchange column of Dionex IonPac AG20 was used as the analytical column with the weakly alkaline mobile phase of 0. 056% ammonia /5 mmol / L Ammonium acetate and the flow rate of 1. 0 mL / min.-ESI MS / MS was used as the detector and the perhclorate was detected under multiple reaction monitoring( MRM) mode. The detection limit of the developed method was 0. 043 μg / L and the linear range was 0. 2-50 μg / L with the linear correlation coefficient of 0. 999 4,the relative standard deviation for about 2,6,30 μg / L sample were 2. 47%,4. 55%,0. 49%,respectively. Content recovery percentages ranging from 80% to 109% were obtained and the analysis results were of agreement with that analyzed by US EPA method314. 0.