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2008•Journal of Sun Yat-sen UniversityRequires access

Effects of Sodium Valproate on Proliferation and Apoptosis of Chronic Myeloid Leukemia Cell Line K562

Yunxian Chen, Sun Yat-sen

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Abstract

【Objective】 To investigate the effects of sodium valproate (VPA) on the proliferation of chronic myeloid leukemia cell line K562,and to explore possible mechanisms. 【Methods】 K562 cells were treated with VPA. Cell proliferation was determined by CCK-8 assay. Cell apoptosis and cell cycle were analyzed by flow cytometry (FCM). 【Results】 VPA inhibited the proliferation of K562 cells in concentration- and time-dependent manners. The apoptosis rate was significantly higher in the cells treated with VPA than in untreated cells[(11.47%±0.25%)vs(4.77%±0.40%), P 0.05]. After treatment of VPA, cell cycle was arrested obviously at G0/ G1 phase (P 0.05). 【Conclusions】 VPA could induce G0/ G1 phase arrest, inhibit the proliferation and induce the apoptosis of K562 cells in vitro.

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What this paper is about

【Objective】 To investigate the effects of sodium valproate (VPA) on the proliferation of chronic myeloid leukemia cell line K562,and to explore possible mechanisms. 【Methods】 K562 cells were treated with VPA. Cell proliferation was determined by CCK-8 assay. Cell apoptosis and cell cycle were analyzed by flow cytometry (FCM). 【Results】 VPA inhibited the proliferation of K562 cells in concentration- and time-dependent manners. The apoptosis rate was significantly higher in the cells treated with VPA than in untreated cells[(11.47%±0.25%)vs(4.77%±0.40%), P 0.05]. After treatment of VPA, cell cycle was arrested obviously at G0/ G1 phase (P 0.05). 【Conclusions】 VPA could induce G0/ G1 phase arrest, inhibit the proliferation and induce the apoptosis of K562 cells in vitro.

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Available abstract

【Objective】 To investigate the effects of sodium valproate (VPA) on the proliferation of chronic myeloid leukemia cell line K562,and to explore possible mechanisms. 【Methods】 K562 cells were treated with VPA. Cell proliferation was determined by CCK-8 assay. Cell apoptosis and cell cycle were analyzed by flow cytometry (FCM). 【Results】 VPA inhibited the proliferation of K562 cells in concentration- and time-dependent manners. The apoptosis rate was significantly higher in the cells treated with VPA than in untreated cells[(11.47%±0.25%)vs(4.77%±0.40%), P 0.05]. After treatment of VPA, cell cycle was arrested obviously at G0/ G1 phase (P 0.05). 【Conclusions】 VPA could induce G0/ G1 phase arrest, inhibit the proliferation and induce the apoptosis of K562 cells in vitro.

Key concepts: Myeloid leukemia, Apoptosis, K562 cells, Cell growth, Flow cytometry, Cell cycle, Cell culture, Chemistry

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Effects of Sodium Valproate on Proliferation and Apoptosis of Chronic Myeloid Leukemia Cell Line K562 — Research Paper | ScholarLens