High-fat diet feeding induces insulin resistance in rats

Aim To establish the model of insulin resistance in rats.Method Male SD rats were fed with high-fat diet,and at 6 weeks after induction,oral glucose tolerance testing(OGTT),insulin tolerance testing(ITT),assays for serum insulin,total cholesterin(TC),triglyceride(TG),and calculation of insulin sensitivity index(ISI)were carried out.Results The weight of rats fed with hight-fat diet was significantly increasing from the begining of the 3th week(P0.05,n=15),and at 6 weeks,OGTT and ITT were significantly abnormal,serum insulin was markedly increased(P0.01,n=15),insulin sensitivity as indicated by ISI significantly decreased(P0.01,n=15),but fasting blood glucose,TC and TG didn't change significantly(P0.05,n=8),compared with control rats Conclusion The characteristics of insulin resistanc was shown when the rats were fed with high-fat diet for 6 weeks.