Transforming Growth Factor-β_1 Stimulates the Proliferation of Rat Lung Fibroblasts in vitro

Objective: To explore stimulates the proliferation of transforming growth factor-β1(TGF-β1) in vitro rat lung fibroblasts.Method: The rat lung fibroblast were cultured in vitro,the 4th generation of cells in logarithmic growth phase were used to proliferation experiments.These cells were randomly divided into five groups: serum-free Dulbecco's modified Eagle medium(DMEM) group,10.0,5.0,2.5,1.25 μg.L-1 of TGF-β1 DMEM group.We selected the optimal stimulation point of TGF-β1 in three different stimulation time points of application(24,48,72 h) by the tetrazolium salt colorimetric method(MTT method) to explore the TGF-β1 stimulating rat lung fibroblast proliferation optimal concentration and the best time point by statistical analysis of variance and multiple comparison.Result: TGF-β1 in 24 h time point directly stimulated the 5 different groups of SD rat lung fibroblast proliferation based on MTT results by analysis of variance,the five groups were no significant difference.In 48 h time point,by the non-parametric test,P 0.05,these groups were significantly different.Seventy-two h time point,by analysis of variance,P 0.05,these groups were significantly different;further two multiple comparisons: control group,10.0,2.5 μg.L-1 group,P 0.05,2.5,1.25 μg.L-1were P 0.01,the rest of the group,P 0.05.Conclusion: 48 h,72 h time point were observed to promote the proliferation of TGFβ1,and the best proliferative activity of stimulate concentration and time point of TGF-β1 were 2.5 μg.L-1,72 h.In this concentration and time point,rat lung fibroblast proliferated significantly.