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2014•Guangxi Medical JournalRequires access

Effects of Leflunomide on Secretion of IL-8 and ICAM-1 in Lipopolysaccharide-Induced HaCaT Cell

Cheng Qiu-me

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Abstract

Objective To observe the effects of leflunomide on the secretion of interleukin 8( IL-8) and intercellular adhesion molecule 1( ICAM-1) in the lipopolysaccharide-induced HaCaT cell. Methods Lipopolysaccharide was used to induce the proliferation of HaCaT cell,supernatants were harvested to detect the levels of IL-8 and ICAM-1 by ELISA. Results The levels of IL-8 and ICAM-1 increased after induction of 1 μg / ml lipopolysaccharide,and decreased after treatment with different concentrations of leflunomide( the concentrations ranged from 30 μg / ml to 270 μg / ml),which was concentration-dependent.Conclusion Leflunomide can inhibit the secretion of IL-8 and ICAM-1 in the lipopolysaccharide-induced HaCaT cell.

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What this paper is about

Objective To observe the effects of leflunomide on the secretion of interleukin 8( IL-8) and intercellular adhesion molecule 1( ICAM-1) in the lipopolysaccharide-induced HaCaT cell. Methods Lipopolysaccharide was used to induce the proliferation of HaCaT cell,supernatants were harvested to detect the levels of IL-8 and ICAM-1 by ELISA. Results The levels of IL-8 and ICAM-1 increased after induction of 1 μg / ml lipopolysaccharide,and decreased after treatment with different concentrations of leflunomide( the concentrations ranged from 30 μg / ml to 270 μg / ml),which was concentration-dependent.Conclusion Leflunomide can inhibit the secretion of IL-8 and ICAM-1 in the lipopolysaccharide-induced HaCaT cell.

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Available abstract

Objective To observe the effects of leflunomide on the secretion of interleukin 8( IL-8) and intercellular adhesion molecule 1( ICAM-1) in the lipopolysaccharide-induced HaCaT cell. Methods Lipopolysaccharide was used to induce the proliferation of HaCaT cell,supernatants were harvested to detect the levels of IL-8 and ICAM-1 by ELISA. Results The levels of IL-8 and ICAM-1 increased after induction of 1 μg / ml lipopolysaccharide,and decreased after treatment with different concentrations of leflunomide( the concentrations ranged from 30 μg / ml to 270 μg / ml),which was concentration-dependent.Conclusion Leflunomide can inhibit the secretion of IL-8 and ICAM-1 in the lipopolysaccharide-induced HaCaT cell.

Key concepts: HaCaT, Lipopolysaccharide, Secretion, Leflunomide, ICAM-1, Chemistry, Intracellular, Pharmacology

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Effects of Leflunomide on Secretion of IL-8 and ICAM-1 in Lipopolysaccharide-Induced HaCaT Cell — Research Paper | ScholarLens