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2004•The Chinese Journal of DermatovenereologyRequires access

The Effect of Mechlorethamine on Cytokines Secretion of Human Keratinocyte HaCaT Cell Line in Vitro

Jun Gu

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Abstract

Objective To Study the effect of mechlorethamine on cytokines secretion of human keratinocyte HaCaT cell line in vitro,both not stimulated and stimulated by PMA/LPS.Methods Expression of IL-1α,IL-2,IL-6 and IL-8 was detected by ELISA kit.Results HaCaT cell secreted IL-1α,IL-2,IL-6 and IL-8 spontaneously;Stimulation by PMA/LPS induced significant increase in IL-6 and IL-8 level and decrease in IL-1α level in HaCaT cell,a pattern similar to those seen in psoriasis.On HaCaT cell,Mechlorethamine increased the secretion of IL-1α,IL-6 and IL-8 distinctly,they may participate in the inflammation of Mechlorethamine on normal skin.On HaCaT cell stimulated with PMA/LPS,Mechlorethamine decreased the secretion of IL-6,IL-8 and increased the secretion of IL-1α in a low concentration,but the secretion of IL-6,IL-8 and IL-1α in stimulated HaCaT cell were significantly increased when Mechlorethamine was in a high concentration.Conclusion These results suggested that the mechanism of Mechlorethamine therapy on psoriasis is related to IL-1α,IL-6 and IL-8.

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Objective To Study the effect of mechlorethamine on cytokines secretion of human keratinocyte HaCaT cell line in vitro,both not stimulated and stimulated by PMA/LPS.Methods Expression of IL-1α,IL-2,IL-6 and IL-8 was detected by ELISA kit.Results HaCaT cell secreted IL-1α,IL-2,IL-6 and IL-8 spontaneously;Stimulation by PMA/LPS induced significant increase in IL-6 and IL-8 level and decrease in IL-1α level in HaCaT cell,a pattern similar to those seen in psoriasis.On HaCaT cell,Mechlorethamine increased the secretion of IL-1α,IL-6 and IL-8 distinctly,they may participate in the inflammation of Mechlorethamine on normal skin.On HaCaT cell stimulated with PMA/LPS,Mechlorethamine decreased the secretion of IL-6,IL-8 and increased the secretion of IL-1α in a low concentration,but the secretion of IL-6,IL-8 and IL-1α in stimulated HaCaT cell were significantly increased when Mechlorethamine was in a high concentration.Conclusion These results suggested that the mechanism of Mechlorethamine therapy on psoriasis is related to IL-1α,IL-6 and IL-8.

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Available abstract

Objective To Study the effect of mechlorethamine on cytokines secretion of human keratinocyte HaCaT cell line in vitro,both not stimulated and stimulated by PMA/LPS.Methods Expression of IL-1α,IL-2,IL-6 and IL-8 was detected by ELISA kit.Results HaCaT cell secreted IL-1α,IL-2,IL-6 and IL-8 spontaneously;Stimulation by PMA/LPS induced significant increase in IL-6 and IL-8 level and decrease in IL-1α level in HaCaT cell,a pattern similar to those seen in psoriasis.On HaCaT cell,Mechlorethamine increased the secretion of IL-1α,IL-6 and IL-8 distinctly,they may participate in the inflammation of Mechlorethamine on normal skin.On HaCaT cell stimulated with PMA/LPS,Mechlorethamine decreased the secretion of IL-6,IL-8 and increased the secretion of IL-1α in a low concentration,but the secretion of IL-6,IL-8 and IL-1α in stimulated HaCaT cell were significantly increased when Mechlorethamine was in a high concentration.Conclusion These results suggested that the mechanism of Mechlorethamine therapy on psoriasis is related to IL-1α,IL-6 and IL-8.

Key concepts: HaCaT, Secretion, Cell culture, Keratinocyte, Psoriasis, Chemistry, In vitro, Interleukin

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The Effect of Mechlorethamine on Cytokines Secretion of Human Keratinocyte HaCaT Cell Line in Vitro — Research Paper | ScholarLens