Preparation of rapeseed peptides from dehulled and cold pressed rapeseed
Dongping He
Abstract
Dongping He
Abstract
Low temperature desolventized meal was obtained by dehulling,coldpressing,extracting and low temperature desolventizing from rapeseed.Rapeseed protein was extracted from the meal,and then was hydrolyzed by neutral protease AS1.398.By orthogonal experiment,the optimum conditions of hydrolysis were as follows:pH 7.2,temperature 45℃,dosage of enzyme 5%,time 2 h.Under the optimum conditions,75% protein was hydrolyzed to peptides.The peptides have some characteristics such as good fluidity,good heat stability and low viscosity.
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Low temperature desolventized meal was obtained by dehulling,coldpressing,extracting and low temperature desolventizing from rapeseed.Rapeseed protein was extracted from the meal,and then was hydrolyzed by neutral protease AS1.398.By orthogonal experiment,the optimum conditions of hydrolysis were as follows:pH 7.2,temperature 45℃,dosage of enzyme 5%,time 2 h.Under the optimum conditions,75% protein was hydrolyzed to peptides.The peptides have some characteristics such as good fluidity,good heat stability and low viscosity.
Key concepts: Rapeseed, Neutral protease, Hydrolysis, Chemistry, Chromatography, Food science, Viscosity, Protease