Aplastic Anemia Mouse Model Established by Combination of Hydracetin,X-ray and Cyclophosphamide

Objective To establish a aplastic anemia mouse model by combination of hydracetin,X-ray and cyclophosphamide.Methods Hydracetin 100 mg/kg was injected subcutaneously on the back of BALB/e mice.Next day, the mice were irradiated by 2.0Gy X-ray,and the fifth day cyclophosphamide 80 mg/kg was injected into abdominal cavity. These steps were repeated the fifteenth day,but the ray treatment was not given.This experiment investigated the hemogram, bone marrow,mitochondria of hematopoietic cells,colony formation of hematopoietic stem cell,as well as the pathological changes of liver and spleen in mice.Results Compared to the control group,the peripheral hemogram of mice in the model group showed a remarkable decrease of three kinds of cells,serum Epo level was much higher,the number of nucleated cells in bone marrow and colony formation of hematopoietic stem cells exhibited significan decrease,with the pathological changes also observed in liver and spleen.Conclusion The present aplastic anemia model,being simple with high successful rate, having persistent pathological changes of peripheral blood and bone marrow,is consistent with clinical situation of aplastic anemia.